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Protein Expression and Purification service
 Recombinant
proteins are used throughout the whole spectrum of biomedical
research. Once you have large quantity of purified specific
protein, you could conduct many downstream experiments, such
as enzyme kinetics, functional and structural studies. AGCT
is pleased to launch the new protein expression and purification
service. AGCT¡¦s protein experts provide the best possible
results to our valued customers. We have the capacity to take
your project directly from gene synthesis to protein expression
and purification in bacterial and mammalian cells. We are
proud of our ability to optimize protein expression, maximize
product yield, and ensure purity to serve every aspect of
your research needs. Amount of the protein will be minimal
2 mg or we could provide any amount as you required with reasonable
additional charge.
Our scientists can deliver high quality purified protein in
a timely manner. We understand that 13C and 15N labeled protein
is very useful for faction and mateibic study in vivo. It
is available to have your protein labeled by such elements.
Starting materials:
It can be from gene sequence to cloned in a cell Or from almost
anywhere you asks us to begin
(1). From Gene to Protein
Starting from de novo gene synthesis, cDNA, BAC clone, we
will synthesis the gene or sub clone the gene into the system
you named.
(2). From a stock to protein
If you have the gene already transformed into a E.Coli, insect cell, or a mammalian cell, you will only pay the protein isolation fee as low as 1980 for minimal 2mg final protein.
Protein Expression Systems available:
1. E.Coli Expression System
Escherichia coli (E. coli) is one of the most widely used
hosts for the production of heterotopous proteins
2. Baculovirus Expression System
The Baculovirus insect cell expression system is widely used
for the production of precisely matured, folded and processed
recombinant proteins. In contrast to E. coli, insect cells
are able to incorporate post-translational modifications.
3. Mammalian Expression System
Mammalian cell lines (HEK, 293 CHO, etc) are widely used for
the production of recombinant glycoproteins (vaccines, enzymes)
as they have the advantage of full posttranslational modifications
of the expressed protein.
* Yeast expression system is available upon request.
Price
and Services Summary
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